风湿

干燥综合征中可检测到AQP-1抗体

作者:Alam J et al 翻译:北医三院金银姬 来源:中国风湿病公众论坛 日期:2017-10-05
导读

         干燥综合征中可检测到AQP-1抗体

关键字:  干燥综合征 | AQP-1抗体 

        摘要

        干燥综合征的腺体功能障碍发病机制尚未完全阐明。之前我们报道了抗水通道蛋白5(AQP-5)抗体存在于干燥综合征患者中,且与低静息唾液流量相关。本研究的目的为明确干燥综合征患者中是否存在抗水通道蛋白1(AQP-1)抗体。使用过表达人AQP1的MDCK细胞,通过细胞间接免疫荧光法检测抗AQP-1抗体。检测112名干燥综合征及52名正常对照组血清。结果显示,27.7%干燥综合征患者中可检测到抗AQP-1抗体,但对照组中未检测到该抗体。有趣的是,抗AQP-1抗体阳性的血清包含抗AQP-5抗体。但与抗AQP-5抗体不同,抗AQP-1抗体与低唾液流率无相关。虽AQP1抗体不能作为诊断标志物,但AQP1自身抗体的存在可能成为干燥综合征患者进行AQP1基因治疗的一个障碍。

        附原文

        ABSTRAT:The pathophysiology of glandular dysfunction in Sjögren's syndrome(SS) has not been fully elucidated. Previously, we reported the presence of autoantibodiesto AQP-5 in patients with SS, which was associated with a low resting salivary flow. The purpose of this study was to investigate the presence of anti-AQP1 autoantibodies. To detect anti-AQP1 autoantibodies, cell-based indirect immunofluorescence assay was developed using MDCK cells that overexpressed human AQP1. By screening 112 SS and 52 control sera, anti-AQP1 autoantibodieswere detected in 27.7% of the SS but in none of the control sera. Interestingly, the sera that were positive for anti-AQP1 autoantibodiesalso contained anti-AQP5 autoantibodiesin the previous study. Different from anti-AQP5 autoantibodies, the presence of anti-AQP1 autoantibodieswas not associated with the salivary flow rate. Although anti-AQP1 autoantibodiesare not useful as a diagnostic marker, the presence of autoantibodiesto AQP1 may be an obstacle to AQP1 gene therapy for SS.

        引自

        Alam J,Choi YS,Koh JH, et al. Detection of Autoantibodiesagainst Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome.Immune Netw.2017 Apr;17(2):103-109. doi: 10.4110/in.2017.17.2.103. Epub 2017 Apr 20.

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